Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Fluorescence imaging in human identity testing

J Worley1, S Lee, M S Ma

  • 1Molecular Dynamics, Sunnyvale, USA. jworley@mdyn.com

Biotechniques
|July 1, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

KIR2DL4-HLAG interaction at human NK cell-oligodendrocyte interfaces regulates IFN-γ-mediated effects.

Molecular immunology·2018
Same author

Walking in the shoes of caregivers of children with obesity: supporting caregivers in paediatric weight management.

Clinical obesity·2017
Same author

Training of Forensic DNA Scientists - A Commentary.

Forensic science review·2015
Same author

FDA oversight of laboratory-developed tests will facilitate adoption of pharmacogenetic testing into routine clinical care.

Journal of clinical pharmacology·2015
Same author

Determination of the surface density of polyethylene glycol on gold nanoparticles by use of microscale thermogravimetric analysis.

Analytical and bioanalytical chemistry·2015
Same author

Evaluating the thermal damage resistance of graphene/carbon nanotube hybrid composite coatings.

Scientific reports·2014

Fluorescence detection using the FluorImager S1 System offers sensitive analysis for human DNA typing. This method enhances the detection of polymerase chain reaction (PCR)-based short tandem repeat (STR) profiles, improving accuracy in genetic analysis.

Area of Science:

  • Molecular Biology
  • Genetics
  • Forensic Science

Background:

  • Accurate human DNA typing is crucial for genetic analysis and forensic applications.
  • Traditional DNA typing methods can face challenges in sensitivity and interpretation.
  • Fluorescence detection offers a promising alternative for enhanced DNA analysis.

Purpose of the Study:

  • To investigate the utility of fluorescence detection with the FluorImager S1 System for human DNA typing.
  • To evaluate different fluorescence-based methods for analyzing DNA typing tests, including Southern hybridization and PCR-based methods.
  • To assess the sensitivity and interpretability of fluorescence detection for short tandem repeat (STR) multiplex allele profiles.

Main Methods:

  • Southern hybridization using alkaline phosphatase-conjugated oligonucleotide probes and chemifluorescent substrates for DNA quantification and locus detection (D2S44, D17Z1).

Related Experiment Videos

  • Testing of fluorescent nucleic acid gel staining and direct fluorescent labeling with fluorescein end-labeled primers for PCR-based D1S80 and STR multiplex analysis.
  • Utilizing denaturing and non-denaturing acrylamide gel electrophoresis for analyzing amplified DNA fragments.

    • Main Results:

    • Fluorescence detection successfully analyzed a comprehensive set of human DNA typing tests.
    • The fluorescent staining method sensitively detected allelic profiles in a rapid 10-min procedure.
    • Fluorescent primers improved STR typing by eliminating doublet band patterns, simplifying interpretation.

    Conclusions:

    • Fluorescence detection with the FluorImager S1 System is a sensitive and effective method for human DNA typing.
    • Fluorescent labeling techniques, particularly with fluorescent primers, enhance the accuracy and ease of interpretation for STR analysis.
    • This approach holds significant potential for advancing genetic analysis and forensic applications.