Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Characterizing human colorectal carcinomas by proteolytic profile

M J Murnane1, S Shuja, E Del Re

  • 1Department of Pathology, Boston University School of Medicine, MA 02118, USA. mmurnane@acs.bu.edu

In Vivo (Athens, Greece)
|May 1, 1997
PubMed
Summary

Researchers identified distinct proteolytic profiles in colorectal cancers using enzyme activity assays. These profiles, based on cathepsin B, L, and matrix metalloproteinase-9 activity, offer prognostic insights beyond traditional staging.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Analysis of perioperative efficacy and safety of cytoreductive surgery in the treatment of colorectal cancer peritoneal metastases].

Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery·2022
Same author

[Medial parapatellar soft tissue overlapping suture in total knee arthroplasty for severe osteoarthritis with permanent patellar dislocation: early and mid-term results].

Nan fang yi ke da xue xue bao = Journal of Southern Medical University·2022
Same author

High efficiency and collimated terahertz pulse from ultra-short intense laser and cone target.

Optics letters·2022
Same author

[Immune-associated encephalopathy after PD-1 inhibitor therapy in a patient with peritoneal metastasis of gastric cancer: a case report].

Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery·2022
Same author

Clinical study of sodium bicarbonated Ringer's solution on fluid resuscitation of patients with hemorrhagic shock.

European review for medical and pharmacological sciences·2022
Same author

[Trend analysis on morbidity and mortality of pancreatic cancer in China, 2005-2015].

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2021

Area of Science:

  • Biochemistry
  • Oncology
  • Molecular Biology

Background:

  • Clinicopathologic staging of colorectal cancer has limitations in predicting patient prognosis.
  • Understanding the molecular mechanisms driving tumor aggressiveness is crucial for improved patient outcomes.

Purpose of the Study:

  • To identify reproducible proteolytic profiles in colorectal carcinomas.
  • To investigate the relationship between proteinase activity and cancer stage.
  • To determine if proteolytic profiles provide prognostic information independent of clinical stage.

Main Methods:

  • Activity assays were performed for cysteine proteinases (cathepsins B, L, H) and matrix metalloproteinases (MMP-2, MMP-9).
  • Proteolytic profiles were characterized based on the combined expression of cathepsin B (CB), cathepsin L (CL), and MMP-9.

Related Experiment Videos

  • Colorectal carcinomas were categorized into four distinct profiles: "Early", "Middle", "Late", and "High".

    • Main Results:

    • Distinct and reproducible proteolytic profiles were identified in colorectal carcinomas.
    • Individual proteinases showed specific activity levels correlating with different cancer stages.
    • Four defined proteolytic profiles could categorize approximately 80% of analyzed colorectal carcinomas.
    • These profiles provided information independent of clinical stage.

    Conclusions:

    • Proteolytic profiles, based on cathepsin and MMP activity, can characterize colorectal cancers.
    • These profiles may reflect non-random steps in tumor development and offer prognostic value.
    • Proteolytic profiling may identify critical variations in tumor behavior beyond current staging methods.