Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

In vitro cytotoxicity of silver-impregnated collagen cuffs designed to decrease infection in tunneled catheters

J B Hemmerlein1, S O Trerotola, M A Kraus

  • 1Department of Radiology, Indiana University School of Medicine, Indianapolis 46202-5253, USA.

Radiology
|August 1, 1997
PubMed
Summary

Silver-impregnated central venous catheter cuffs show cytotoxicity to human fibroblasts in vitro, creating cell-free zones. This may explain clinical issues with catheter anchorage and removal.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Health professionals and students encounter multi-level barriers to implementing high-value osteoarthritis care: a multi-national study.

Osteoarthritis and cartilage·2019
Same author

Epigenetic variation at the SLC6A4 gene promoter in mother-child pairs with major depressive disorder.

Journal of affective disorders·2018
Same author

Comparing vegetation types and anthropic disturbance levels in the Atlantic forest: how do Pentatomoidea (Hemiptera: Heteroptera) assemblages respond?

Environmental entomology·2014
Same author

Dosimetry of very high energy electrons (VHEE) for radiotherapy applications: using radiochromic film measurements and Monte Carlo simulations.

Physics in medicine and biology·2014
Same author

Morphoanatomy of the flower of Syagrus inajai (SPRUCE) Becc. (Arecaceae- Arecoideae- Attaleinae), Amazon.

Brazilian journal of biology = Revista brasleira de biologia·2013
Same author

Change in the pattern of adrenal venous sampling over time in patients with primary aldosteronism.

Journal of human hypertension·2008

Area of Science:

  • Biomaterials science
  • Cell biology
  • Medical device research

Background:

  • Central venous catheters (CVCs) are crucial for patient care.
  • Silver impregnation is used in CVCs to prevent infections.
  • The effect of silver-impregnated materials on host cells requires investigation.

Purpose of the Study:

  • To evaluate the in vitro cytotoxic effects of silver-impregnated collagen cuff material from CVCs on human fibroblast growth.

Main Methods:

  • Human fibroblasts and hybrid cells were cultured with silver-impregnated or silver-free collagen cuff material.
  • Cells were incubated for 72 hours.
  • Cytotoxicity was assessed by measuring cell-free zones using digital imaging.

Main Results:

Related Experiment Videos

  • Silver-impregnated collagen cuff material induced significant local cytotoxicity in both hybrid cells and human fibroblasts.
  • A mean cell-free area of 312 mm² was observed around the silver-impregnated cuffs with human fibroblasts (P < .0001).
  • No cytotoxic effect was observed with silver-free control cuffs.

Conclusions:

  • Silver-impregnated collagen cuff material exhibits in vitro cytotoxicity towards human fibroblasts.
  • This cytotoxicity may contribute to clinical observations of reduced catheter anchorage and inadvertent removal.
  • Further research is warranted to understand the clinical implications of this finding.