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A comparison of murine and human factor XI
1Department of Pathology, Vanderbilt University School of Medicine, Nashville, TN 37232-6350, USA.
Blood
|August 1, 1997
Summary
Researchers developed a mouse model for factor XI deficiency, finding murine factor XI shares structural and enzymatic similarities with human factor XI, but with species-specific expression and activity differences.
Area of Science:
- Biochemistry
- Molecular Biology
- Hematology
Background:
- Factor XI (FXI) is crucial for blood coagulation and hemostasis.
- Understanding FXI's role requires appropriate animal models for deficiency studies.
Purpose of the Study:
- To clone and characterize murine factor XI (FXI) for a novel mouse model of FXI deficiency.
- To compare the properties of recombinant murine FXI with human FXI.
Main Methods:
- Cloning of murine FXI cDNA and expression in a mammalian system.
- Biochemical characterization of recombinant murine and human FXI.
- Analysis of FXI gene expression using Northern blots.
Main Results:
- Murine FXI cDNA encodes a 624-amino acid protein with 78% homology to human FXI.
- Recombinant murine FXI functions as a 160 kD homodimer, similar to human FXI.
- Murine FXI exhibits 50-70% specific activity compared to human FXI and differs in autoactivation mechanisms and tissue expression patterns.
Conclusions:
- Murine and human FXI share significant structural and enzymatic similarities.
- Subtle differences in enzyme activity and tissue-specific expression exist between murine and human FXI.
- The characterized murine FXI provides a foundation for developing a factor XI deficiency mouse model.