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Updated: Jul 27, 2026
09:02
Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
Published on: January 8, 2015
Subtractive cloning: past, present, and future
C G Sagerström1, B I Sun, H L Sive
1Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA.
Annual Review of Biochemistry
|January 1, 1997
Summary
Subtractive cloning isolates specific genes by comparing cell populations. This review covers its history, current methods like cDNA library and PCR-based techniques, and future directions in gene discovery.
Area of Science:
- Molecular Biology
- Genetics
- Biotechnology
Background:
- Subtractive cloning identifies differentially expressed genes.
- Origins trace back to nucleic acid reassociation techniques.
- Positive selection is a related gene isolation method.
Purpose of the Study:
- To review the history and evolution of subtractive cloning.
- To explore current subtractive cloning and positive selection techniques.
- To discuss future advancements in gene isolation methods.
Main Methods:
- Historical review of nucleic acid reassociation.
- Analysis of cDNA library-based subtractive techniques.
- Evaluation of PCR-based subtractive cloning methods.
Main Results:
- Detailed historical context of subtractive techniques.
- Comparative analysis of current subtractive cloning approaches.
- Identification of strengths and weaknesses of various methods.
Conclusions:
- Subtractive cloning remains a vital tool for gene discovery.
- Current methods offer diverse strategies for isolating specific genes.
- Emerging techniques promise enhanced efficiency and broader applications.
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