Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Direct preparation of cyclodextrin monophosphates

E Tarelli1, X Lemercinier, S Wheeler

  • 1Joint Microbiology Research Unit, Kings College School of Medicine and Dentistry, London, UK. rcbm800@kcl.ac.uk

Carbohydrate Research
|July 11, 1997
PubMed
Summary

Researchers developed a simple method to create and separate isomeric monophosphate esters using cyclodextrins and metaphosphate. This technique offers a streamlined route to these valuable chemical derivatives.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Compensatory changes after spinal cord injury in a remyelination deficient mouse model.

Journal of neurochemistry·2024
Same author

[Prevalence of visual impairment in school-age children: Data analysis from PlanVue® pilot project].

Journal francais d'ophtalmologie·2021
Same author

Hendra in the Hunter Valley.

One health (Amsterdam, Netherlands)·2020
Same author

Reduced voltage losses yield 10% efficient fullerene free organic solar cells with >1 V open circuit voltages.

Energy & environmental science·2017
Same author

Chromosomal microarray provides enhanced targetable gene aberration detection when paired with next generation sequencing panel in profiling lung and colorectal tumors.

Cancer genetics·2016
Same author

A method comparison of a food frequency questionnaire to measure folate, choline, betaine, vitamin C and carotenoids with 24-h dietary recalls in women of reproductive age.

European journal of clinical nutrition·2015

Area of Science:

  • Carbohydrate Chemistry
  • Analytical Chemistry
  • Biochemistry

Background:

  • Cyclodextrins are cyclic oligosaccharides with unique host-guest properties.
  • Monophosphate esters are important in various biological and chemical applications.
  • Current synthesis and separation methods can be complex and time-consuming.

Purpose of the Study:

  • To develop a novel and efficient method for synthesizing isomeric monophosphate esters.
  • To establish a facile separation technique for these ester mixtures.
  • To characterize the synthesized derivatives thoroughly.

Main Methods:

  • Reaction of cyclodextrins with inorganic metaphosphate in aqueous solution at pH 4.
  • Drying and subsequent warming to induce ester formation.

Related Experiment Videos

  • Separation of isomeric products using anion-exchange chromatography.
  • Characterization via enzymatic assays, mass spectrometry, and NMR spectroscopy.

    • Main Results:

    • A mixture of isomeric monophosphate esters was successfully produced.
    • The products were effectively separated by anion-exchange chromatography.
    • Comprehensive characterization confirmed the identity and isomeric nature of the products.
    • The methodology represents a single-step synthesis and separation route.

    Conclusions:

    • Aqueous solutions of cyclodextrins and inorganic metaphosphate provide a viable route to isomeric monophosphate esters.
    • Anion-exchange chromatography is an effective method for separating these isomers.
    • This unified approach simplifies the preparation and purification of monophosphate ester derivatives.