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Acute experimental allergic encephalomyelitis. Myelin basic protein-reactive T cells in the circulation and in
Abstract:
MBP-reactive early T cells were determined simultaneously in the circulation and in inflammatory cells from the meninges of guinea pigs with acute EAE. In addition, early and late T cells as well as B cells were estimated in both compartments. Within the circulation, early T cells were found to decrease at the onset of clinical signs, and MBP-reactive early T cells were significantly higher than in normals. Different changes were found in inflammatory cells from the meninges. In this cell population, early T cells were increased but did not show significant, further elevation after incubation with MBP. This may indicate that the majority of early T cells in meningeal infiltrates had been previously activated by MBP in vivo.
Insights
Early T cells in the circulation decrease during EAE onset, while MBP-reactive T cells increase. Meningeal T cells rise but don
Area of Science:
- Immunology
- Neuroscience
- Cell Biology
Background:
- Experimental autoimmune encephalomyelitis (EAE) is an animal model for multiple sclerosis.
- T cells play a crucial role in the pathogenesis of EAE.
Purpose of the Study:
- To investigate the dynamics of early T cells and MBP-reactive T cells in the circulation and meninges during acute EAE.
- To differentiate between circulating and locally activated T cells in the central nervous system.
Main Methods:
- Quantification of early T cells, late T cells, and B cells in both circulation and meningeal inflammatory cells.
- Assessment of MBP-specific T cell responses in vivo and in vitro.
Main Results:
- Circulating early T cells decreased at clinical onset, while MBP-reactive early T cells were elevated compared to controls.
- Meningeal early T cells increased, but did not show further MBP-induced proliferation in vitro, suggesting prior in vivo activation.
Conclusions:
- Early T cell dynamics differ between circulation and the inflamed CNS during EAE.
- Meningeal infiltrates likely contain a significant population of MBP-activated T cells prior to their detection in the inflamed tissue.