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Related Experiment Videos

Multianalyte biosensors on optical imaging bundles

B G Healey1, L Li, D R Walt

  • 1Max Tishler Laboratory for Organic Chemistry, Department of Chemistry, Tufts University, Medford, Massachusetts 02155, USA.

Biosensors & Bioelectronics
|January 1, 1997
PubMed
Summary

This study introduces novel dual-analyte optical biosensors for simultaneous measurement of target analytes and their associated transducing analytes. This innovation enhances enzyme biosensor capabilities, enabling accurate quantification during complex biological processes like fermentation.

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Area of Science:

  • Biomedical Engineering
  • Chemical Sensors
  • Optical Biosensing

Background:

  • Enzyme biosensors are crucial for analyte detection but can be limited by interfering factors.
  • Simultaneous measurement of multiple analytes is challenging with conventional biosensor designs.

Purpose of the Study:

  • To develop a novel optical biosensor design for simultaneous, independent measurement of two analytes.
  • To expand the utility of enzyme biosensors through a new fabrication method.

Main Methods:

  • Fabrication of dual-analyte optical biosensors using site-selective photodeposition of analyte-sensitive polymer matrices on optical imaging fibers.
  • Development of integrated optical-biosensors capable of measuring both dependent and independent analytes concurrently.
  • Demonstration of sensor functionality with penicillin/pH and glucose/O2 dual-analyte systems.

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Main Results:

  • Successfully prepared integrated optical-biosensors for simultaneous measurement of penicillin/pH and glucose/O2.
  • Achieved independent quantification of penicillin (0.25-10.0 mM) across a pH range (6.2-7.5) and glucose (0.6-20.0 mM) across an O2 range (20-100%).
  • Validated the sensor's utility by quantifying penicillin production during a Penicillium chrysogenum fermentation.

Conclusions:

  • The novel dual-analyte optical biosensor design significantly enhances enzyme biosensor capabilities.
  • This technology allows for accurate analyte quantification in the presence of interfering environmental changes.
  • The demonstrated application in fermentation monitoring highlights the practical utility of this advanced biosensing approach.