Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Quantitative reverse transcriptase-polymerase chain reaction for prostate-specific antigen mRNA

B Galvan1, T K Christopoulos

  • 1Department of Chemistry and Biochemistry, University of Windsor, Ontario, Canada.

Clinical Biochemistry
|July 1, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Micafungin at a standard dosage of 100 mg/day achieves adequate plasma exposure in critically ill patients with severe burn injuries.

Intensive care medicine·2014
Same author

[Epidural hematoma secondary to epidural catheter for analgesia in a polytraumatized patient].

Medicina intensiva·2006
Same author

[Relation between clinical symptoms and the results of a computerized dynamic posturography].

Acta otorrinolaringologica espanola·2002
Same author

Enzyme-amplified aequorin-based bioluminometric hybridization assays.

Analytical chemistry·2001
Same author

Expression immunoassay.

Methods (San Diego, Calif.)·2000
Same author

Expression hybridization assays combining cDNAs from firefly and Renilla luciferases as labels for simultaneous determination of two target sequences.

Analytical chemistry·2000
Same journal

Analytical outlier rates for Roche fifth generation cardiac troponin T stat using biotin sensitive and insensitive reagent lots.

Clinical biochemistry·2026
Same journal

Evaluation of hemoglobin interference thresholds for chemical urinalysis panels.

Clinical biochemistry·2026
Same journal

Integrated genomic and biochemical diagnosis of a novel homozygous start-loss variant in AKR1D1 associated with neonatal cholestasis.

Clinical biochemistry·2026
Same journal

Agreement between POC glucose meters and blood gas analyzers varies across ICU patient populations.

Clinical biochemistry·2026
Same journal

From variability to value: Advancing vancomycin therapeutic drug monitoring toward decision-grade practice through commutability-informed external quality assessment and uncertainty-aware reporting.

Clinical biochemistry·2026
Same journal

Response to the letter to the editor: From variability to value: Advancing vancomycin therapeutic drug monitoring toward decision-grade practice through commutability-informed external quality assessment and uncertainty-aware reporting.

Clinical biochemistry·2026
See all related articles

A new quantitative assay accurately measures prostate-specific antigen (PSA) mRNA levels. This method aids in monitoring prostate cancer progression by quantifying PSA mRNA in patient samples.

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Oncology

Background:

  • Prostate-specific antigen (PSA) mRNA is a key biomarker for prostate cancer.
  • Accurate quantification of PSA mRNA is crucial for monitoring disease progression and treatment response.
  • Existing methods may lack the sensitivity or specificity required for precise monitoring.

Purpose of the Study:

  • To develop and validate a quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) assay for monitoring prostate-specific antigen (PSA) mRNA.
  • To establish a reliable method for assessing PSA mRNA levels in the context of prostate cancer research.

Main Methods:

  • Developed a quantitative RT-PCR assay measuring PSA mRNA in parallel with beta-actin mRNA.
  • Incorporated digoxigenin-dUTP during PCR for subsequent detection.

Related Experiment Videos

  • Utilized time-resolved immunofluorometric hybridization assays with specific probes and an anti-digoxigenin antibody.
  • Measured fluorescence after substrate reaction with alkaline phosphatase for quantification.

    • Main Results:

    • The assay demonstrated linearity in hybridization assays within the 1.4-110 pmol/L range.
    • PCR amplification showed exponential phase up to 200,000 PSA and 100,000 actin cDNA molecules.
    • A linear relationship was observed between fluorescence ratios and the number of LNCaP cells (20-3000 cells).
    • Reproducibility studies showed coefficients of variation (CVs) between 11.8% and 14.7% for fluorescence ratios.

    Conclusions:

    • A quantitative methodology for monitoring PSA mRNA has been successfully developed.
    • This assay is expected to be valuable for studying prostate cancer spread and progression.
    • The assay provides a sensitive and reproducible tool for PSA mRNA quantification.