[A fully enzymatic triglyceride determination in a continuous flow 12-channel analyzer (author's transl)]

Area of Science:

• Biochemistry
• Clinical Chemistry
• Analytical Chemistry

Context:

• Triglyceride determination is crucial for diagnosing and monitoring various metabolic disorders.
• Existing methods may have limitations in terms of speed, precision, or automation.
• Adaptation of enzymatic assays to automated analyzers is essential for high-throughput clinical laboratories.

Purpose:

• To adapt and validate a fully enzymatic triglyceride determination method for a continuous flow 12-channel analyzer.
• To assess the linearity, precision, and accuracy of the adapted method.
• To compare the results with a well-established manual method.

Summary:

• A fully enzymatic method for triglyceride determination, involving lipase-esterase hydrolysis and subsequent glycerol measurement, was successfully adapted to a 12-channel continuous flow analyzer.
• The method demonstrated good linearity up to 7.9 mmol/l, with analytical precision (RSD) ranging from 0.8% to 7.2% across different concentration ranges under routine conditions.
• No interference was observed from bilirubin, and comparisons with the manual Eggstein & Kreutz method showed a consistent underestimation corrected by an additive factor, with accuracy controls yielding a 10% difference.

Impact:

• Provides a robust, automated, and precise method for triglyceride quantification in clinical diagnostics.
• Facilitates high-throughput analysis of lipid profiles, aiding in the efficient diagnosis and management of dyslipidemia.
• Offers a reliable alternative to manual methods, improving laboratory workflow and potentially reducing turnaround times.