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Purification of active Escherichia coli ribosome recycling factor (RRF) from an osmo-regulated expression system
J MacDougall1, P Holst-Hansen, K K Mortensen
1CNRS-UPR 9073, Institut de Biologie Physico-chimique, Paris, France.
Biochimie
|May 1, 1997
Abstract:
Ribosome release factor (RRF) from Escherichia coli was overproduced from an osmo-expression vector. More than 40% of cell protein was RRF after 6 h of induction. A purification scheme is described that produced 50 mg of RRF from an initial culture of 2 L. The recycling time for ribosomes synthesising the tripeptide fMet-Phe-Leu in vitro in the absence of RF3 was reduced from 40 to 15 s by the addition of purified 1.5 microM RRF.