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Updated: Jul 31, 2026

Hydrodynamic Renal Pelvis Injection for Non-viral Expression of Proteins in the Kidney
Published on: January 8, 2018
Rat kidney thromboxane synthase: cDNA cloning and gene expression regulation in hydronephrotic kidney
E Tsutsumi1, K Takeuchi, T Abe
1Second Department of Internal Medicine, Tohoku University School of Medicine, Sendai, Japan.
Abstract:
We isolated a rat homolog of thromboxane (TX) synthase cDNA (-1.8 kb) from the kidney with a fragment of human TX synthase cDNA amplified by polymerase chain reaction with placenta cDNA as a template. Northern blot analysis has shown that rat TX synthase gene is expressed abundantly in lung, liver, and uterus; moderately in kidney. TX synthase mRNA expression was up-regulated in hydronephrotic kidney made by ureter ligation. In conclusion, we have revealed the structure of rat kidney TX synthase. Up-regulation of renal TX synthase, which may cause stimulation of TX synthesis, is possibly implicated in the tissue injury in hydronephrotic kidney.
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