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Related Experiment Videos

The electrogenic Na/HCO3 cotransporter

W F Boron1, P Fong, M A Hediger

  • 1Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut, USA.

Wiener Klinische Wochenschrift
|June 27, 1997
PubMed
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Researchers cloned the salamander electrogenic sodium bicarbonate cotransporter (NBC), a key protein in kidney tubules that regulates pH. This discovery identified a new family of transporters crucial for cellular and blood pH balance.

Area of Science:

  • Physiology
  • Molecular Biology
  • Biochemistry

Background:

  • The electrogenic Na/HCO3 cotransporter (NBC) is vital for pH regulation in renal proximal tubules.
  • NBCs are crucial for maintaining intracellular and blood pH homeostasis.
  • Previous studies identified NBCs in various tissues, but molecular cloning was pending.

Purpose of the Study:

  • To expression clone the salamander renal electrogenic Na Bicarbonate Cotransporter (NBC).
  • To characterize the function of the cloned NBC using Xenopus laevis oocytes.
  • To identify novel families of bicarbonate transporters.

Main Methods:

  • Expression cloning using Xenopus laevis oocytes injected with RNA from Ambystoma tigrinum.
  • Monitoring membrane potential (Vm) and intracellular pH (pHi) using microelectrodes.

Related Experiment Videos

  • Functional assays involved CO2/HCO3- application and Na+ removal, with DIDS as a specific blocker.

    • Main Results:

    • Successfully expression cloned the salamander NBC (aNBC) and a rat homolog (rNBC).
    • aNBC-expressing oocytes showed significant membrane potential changes and pHi regulation in response to CO2/HCO3- and Na+ removal.
    • aNBC represents the first member of a new family of Na(+)-linked HCO3- transporters.

    Conclusions:

    • The cloned aNBC is a functional electrogenic Na/HCO3 cotransporter.
    • This discovery expands the known superfamily of bicarbonate transporters.
    • NBCs play a critical role in renal physiology and pH homeostasis.