Related Experiment Video
Updated: Aug 1, 2026

Identification of Functional Protein Regions Through Chimeric Protein Construction
Published on: January 8, 2019
Complementary oligonucleotides and the origin of the mammalian involucrin gene
1School of Medicine, University of Pennsylvania, Department of Dermatology, Philadelphia, USA. htsengpe@mail.med.upenn.edu
Abstract:
A large portion of the consensus sequence of four mammalian involucrin genes comprises four pairs of complementary oligonucleotides. One of the oligonucleotides in each pair is rich in purines and the other in pyrimidines. The perfect complementarity of a number of pairs apparently resulted from a concerted single nucleotide substitution in each pairing oligonucleotide from their respective consensus. This suggests that one member of the pair was derived from the other member of the same pair by a template-dependent replication mechanism. Such a replication mechanism differs from the one that creates tandem repeats by producing short complementary sequence on the same strand of DNA.
Related Concept Videos
In-vitro Mutagenesis
Complementary DNA
Homologous Recombination
Non-LTR Retrotransposons
Leaky Scanning
Exon Recombination
Exon shuffling follows “splice frame rules.” Each exon has three reading...

