Hydrogen exchange at equilibrium: a short cut for analysing protein-folding pathways?

¹Centre for Protein Engineering, MRC Centre, Cambridge, UK.

|August 1, 1997

Abstract:

Hydrogen exchange is an attractive method for observing small populations of partly unfolded states of proteins at equilibrium. It has been suggested that these represent folding intermediates so that hydrogen exchange can offer a short cut for studying protein-folding pathways. This cannot work in theory because it is not possible to tell whether they are intermediates or side reactions. Experimental studies of barnase and chymotrypsin inhibitor 2 show that there is no obvious relationship between hydrogen exchange at equilibrium and their folding pathways.

Related Concept Videos

Articles linked to this work by shared authors, journal, and citation graph.

Molecular basis of S100 proteins interacting with the p53 homologs p63 and p73.

Oncogene·2010

The accessory subunit of mitochondrial DNA polymerase gamma determines the DNA content of mitochondrial nucleoids in human cultured cells.

Nucleic acids research·2009

Regulation by phosphorylation of the relative affinities of the N-terminal transactivation domains of p53 for p300 domains and Mdm2.

Oncogene·2009

Related Experiment Videos

Hydrogen exchange at equilibrium: a short cut for analysing protein-folding pathways?

Related Concept Videos

Molecular basis of S100 proteins interacting with the p53 homologs p63 and p73.

The accessory subunit of mitochondrial DNA polymerase gamma determines the DNA content of mitochondrial nucleoids in human cultured cells.

Regulation by phosphorylation of the relative affinities of the N-terminal transactivation domains of p53 for p300 domains and Mdm2.

Time-resolved fluorescence resonance energy transfer study shows a compact denatured state of the B domain of protein A.

Structure-function-rescue: the diverse nature of common p53 cancer mutants.

Solution structure of a protein denatured state and folding intermediate.

The expanding functional landscape of alternative splicing in plants.

RAF-like protein kinase signaling modules control plant proton pump activity.

Telomeres and telomerase: revealing the end game.

Practical tools for building neuroinclusive research environments.

Driving scientific discovery through neurodivergent cognitive strengths.

Building scientific communities for the future of the molecular life sciences: The role of FEBS.

Exploring the Boundaries of Aromaticity in Complex Structures and Excited States Through Computational Analysis.

Determination of coverage-dependent surface reaction rates using λ-dynamics: Application to molecular desorption.

Environmental effects in the vibrational shifts of water: From the monomer to models of the bulk.

Topological contribution to the split first peak of the liquid water structure factor using the MACE machine-learning potential.

Why Choline Chloride? A Comparative Study of Physicochemical Properties and Machine Learning-Driven Insights Into Melting Point Trends.

Explicit Solvation Effects on the Redox Thermodynamics of Fe(III)/Fe(II)-Quercetin Complexes: A DFT/MD Study.

Related Experiment Videos

Hydrogen exchange at equilibrium: a short cut for analysing protein-folding pathways?

Abstract:

Related Concept Videos

Related Articles