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Micellar electrokinetic capillary chromatography with laser-induced fluorimetric detection of amines in beer
L M Preston1, M L Weber, G M Murray
1Department of Chemistry and Biochemistry, University of Maryland Baltimore County, Baltimore 21250, USA.
Abstract:
Capillary electrophoresis with simultaneous ultraviolet absorbance and laser-induced fluorescence detection is applied to identify and quantify selected amines in beer following derivatization with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole. Quantitation was performed using the method of standard addition in order to avoid pH-dependent variations in the reactivity of the derivatizing agent with the added benefit of verifying peak identity. An inexpensive and "easy-to-use" on-column fiber optic fluorescence detection cell is described and implemented in the analysis.