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Updated: Aug 13, 2026

Purification and Visualization of Lipopolysaccharide from Gram-negative Bacteria by Hot Aqueous-phenol Extraction
Published on: May 28, 2012
R-form lipopolysaccharides (LPS) of Gram-negative bacteria as possible vaccine antigens
E S Stanislavsky1, T A Makarenko, E V Kholodkova
1I.I. Mechnikov Research Institute for Vaccines and Sera, Moscow, Russia.
Abstract:
The antigenic and immunogenic properties of R-form lipopolysaccharides (LPS) of Pseudomonas aeruginosa, Salmonella spp., Escherichia coli and Shigella spp. were studied. The results showed the presence of antigenic relationships among P. aeruginosa R mutants with different structures of the LPS core lipid A region and also among E. coli, Shigella and P. aeruginosa R-LPS, but not with S. minnesota Re-LPS. Vaccines prepared with R-LPS proved to be effective preparations for the active immunization of mice against P. aeruginosa infection. The vaccine stimulated 40-100% protection in mice depending upon the scheme of immunization.
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