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A preliminary study on immune response to hepatitis E virus DNA vaccine in mice
Objective:
In order to elicit immune response to hepatitis E virus (HEV) with the method of direct injection of naked DNA.
Methods:
The gene encoding the structural protein of pORF3, a Chinese strain of HEV, was cloned into the eukaryotic expression plasmid pSVL. The resulted plasmid pSVL-HEV ORF3 was used as HEV DNA vaccine, to inoculate Balb/c mice intramuscularly with a dose of 100 micrograms. Mice injected with empty pSVL DNA served as control.
Results:
After 1-2 times inoculation, twelve of 16 mice inoculated with pSVL-HEV ORF3 had anti-HEV IgG seroconversion. pSVL-HEV ORF3 was still detectable in the muscle of the inoculated mice 18 days after the injection, by the method of PCR. None of the control group had a detectable level of anti-HEV IgG. It is also found that the humoral immune response to HEV induced by DNA vaccine could be boostered by HEV recombinant fusion protein.
Conclusion:
Our present study demonstrated that nucleic acid vaccine is able to prime a specific humoral immune response to HEV in mice.