Glucose affinity for the glucose transporter Glut1 in native or reconstituted lipid bilayers. Temperature-dependence...

The affinity of D-glucose and the transport inhibitor cytochalasin B (CB) for the glucose transporter Glut1 was studied at 5-42 degrees C by quantitative frontal affinity chromatography on sterically immobilized human red cell membrane vesicles, and on proteoliposomes containing reconstituted red cell membrane proteins. Glut1 in the vesicles showed the highest glucose affinity; the dissociation constant Kd(glc) was nearly constant (16 +/- 3 mM) from 15 degrees C to 37 degrees C. For Glut1 in proteoliposomes Kd(glc) decreased from 56 mM at 5 degrees C to 26 mM at 42 degrees C. The CB-Glut1 affinity was strongest around 20 degrees C and was mostly higher with the vesicles, Kd (CB) being 49 nM at 19 degrees C. The entropy and entropy and enthalpy changes for the interactions were calculated.