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Distinct type-2A protein phosphatases activate HMGCoA reductase and acetyl-CoA carboxylase in liver
V Gaussin1, P Skarlas, Y P Ching
1Hormone and Metabolic Research Unit, International Institute of Cellular and Molecular Pathology, Louvain University Medical School, Brussels, Belgium.
Abstract:
Acetyl-CoA carboxylase and HMGCoA reductase are inactivated by the same AMP-activated protein kinase and are activated by type-2A protein phosphatase. To determine whether the same species of protein phosphatase-2A were involved, we studied the interconversion of acetyl-CoA carboxylase and HMGCoA reductase in isolated rat hepatocytes. We show that (i) these enzymes are differently regulated in hepatocytes and (ii) the species of type-2A protein phosphatase involved in their activation are different and can be separated by anion-exchange chromatography.