Anionic sites of charge barrier in the guinea pig crista ampullaris

In present studies we obtained anionic sites in the epithelial and capillary basement membranes in the dark cell area of the crista ampullaris in the guinea pig. The immersion method with cationic tracer polyethyleneimine (PEI) was applied. Electronmicroscopically, the arrangement of PEI particles was observed as two strata along the basement membrane. The number of particles could be counted and compared in each portion. The control test with protamine sulfate showed that the number of PEI particles decreased in both the epithelium and capillaries of the dark cell area. In the experiment using furosemide, the stria vascularis and the dark cells had changed, with pathological findings of interstitial edema and PEI particles reduced in number. It is suggested that the PEI particles reflect different conditions of charge in the basement membrane, which influences the production or absorption of the inner ear fluid.