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Related Experiment Videos

Rice molecular genetic map using RFLPs and its applications

Y Nagamura1, B A Antonio, T Sasaki

  • 1National Institute of Agrobiological Resources/Institute of Society for Techno-innovation of Agriculture, Forestry and Fisheries, Ibaraki, Japan.

Plant Molecular Biology
|September 18, 1997
PubMed
Summary

Significant advancements in rice molecular genetic mapping have been achieved using over 3000 DNA markers, primarily Restriction Fragment Length Polymorphisms (RFLPs). This progress solidifies rice

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Area of Science:

  • Plant Genomics
  • Molecular Genetics
  • Agricultural Science

Background:

  • Rice molecular genetic mapping has seen substantial progress over the last decade.
  • Over 3000 DNA markers, predominantly Restriction Fragment Length Polymorphisms (RFLPs), have been mapped onto the rice genome.
  • Rice is recognized as a pivotal cereal crop and a model organism for genome analysis, alongside Arabidopsis.

Purpose of the Study:

  • To review the current status of rice molecular genetic map construction.
  • To summarize the diverse applications of these genetic maps in rice research.
  • To highlight rice's role as a model plant in cereal genomics.

Main Methods:

  • Utilizing genomic or cDNA clones for DNA marker development.
  • Employing Restriction Fragment Length Polymorphisms (RFLPs) as primary markers.

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  • Developing high-density molecular genetic maps.

    • Main Results:

    • Mapping of over 3000 DNA markers on the rice genome.
    • Advancement in tagging genes of interest and gene isolation via map-based cloning.
    • Progress in comparative mapping across different cereal genomes.

    Conclusions:

    • Rice molecular genetic maps are well-established and continuously improving.
    • These maps are crucial for gene discovery, crop improvement, and understanding cereal genome evolution.
    • Rice's status as a model plant is reinforced by the extensive genomic resources available.