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Rice transformation: bombardment

P Christou1

  • 1Laboratory for Transgenic Technology & Metabolic Pathway Engineering, John Innes Center, Norwich, UK.

Plant Molecular Biology
|September 18, 1997
PubMed
Summary
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Particle bombardment effectively genetically modifies rice, enabling the development of transgenic plants with stable gene expression for traits like pest resistance. This technology supports field trials and commercialization of improved rice varieties.

Area of Science:

  • Plant Molecular Biology
  • Genetic Engineering
  • Agricultural Science

Background:

  • Particle bombardment is a key technology for genetic modification in major cereals like rice.
  • Significant progress has been made in rice molecular biology and genetic engineering using bombardment procedures.

Purpose of the Study:

  • To summarize and discuss bombardment technology for rice genetic engineering.
  • To explore its applications, including field trials and commercialization of transgenic rice.

Main Methods:

  • Utilizing particle bombardment for direct DNA transfer into rice tissues.
  • Molecular and genetic characterization of over 500 independent transgenic rice plants.
  • Evaluating transgene structure, expression, and stability across multiple generations in greenhouse and field settings.

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Main Results:

  • Particle bombardment allows for the recovery of numerous independently derived transgenic rice plants in a variety-independent manner.
  • Transgene expression stability depends on promoter, transgene, and sometimes gene copy number.
  • Comparison of gene copy number, expression level, and stability with other DNA delivery methods, including Agrobacterium-mediated gene transfer.

Conclusions:

  • Bombardment-based methods are effective for rice genetic manipulation, yielding stable transgenic events.
  • The technology has potential for developing commercially viable transgenic rice with desirable agronomic traits.
  • Further research and field trials are warranted for the advancement and application of this technology.