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Candidin: comparison of two antigens for cutaneous delayed hypersensitivity testing
C Fava-Netto1, W Gambale, J Croce
1Instituto de Ciências Biomédicas, Universidade de São Paulo (USP).
Revista Do Instituto De Medicina Tropical De Sao Paulo
|November 1, 1996
Summary
This study compared Candida albicans yeast cell suspension and polysaccharide antigens for delayed hypersensitivity skin testing. The polysaccharide antigen is a viable alternative for evaluating cell-mediated immunity to Candida.
Area of Science:
- Immunology
- Mycology
Background:
- Candidin, a killed yeast cell suspension, is a standard antigen for intradermal delayed hypersensitivity testing.
- Histopathology of positive candidin skin tests shows characteristic hypersensitivity reactions and neutrophilic abscesses.
Purpose of the Study:
- To compare the efficacy of a polysaccharide antigen derived from Candida albicans with the traditional yeast cell suspension antigen.
- To determine if the polysaccharide antigen can be reliably used for intradermal delayed hypersensitivity testing.
Main Methods:
- Intradermal skin tests were performed on 100 individuals using both Candida albicans polysaccharide antigen and yeast cell suspension antigen.
- Results were analyzed for positivity rates and concordance between the two antigen types.
Main Results:
- The yeast cell suspension antigen showed a positivity rate of 69.0%, while the polysaccharide antigen yielded 61.0%.
- Concordant results between the two antigens were observed in 82.0% of the individuals tested.
Conclusions:
- The polysaccharide antigen from Candida albicans demonstrates potential for use in intradermal delayed hypersensitivity testing.
- This antigen offers a comparable alternative to yeast cell suspensions for assessing cell-mediated immunity to Candida.