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Brain-derived neurotrophic factor in human platelets

F B Pliego-Rivero1, N Bayatti, X Giannakoulopoulos

  • 1Department of Biochemistry, Imperial College of Science, Technology and Medicine, London, UK.

Biochemical Pharmacology
|July 1, 1997
PubMed
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A new assay accurately measures brain-derived neurotrophic factor (BDNF) in human platelets. This method uses minimal blood and is suitable for studying clinical disorders.

Area of Science:

  • Biochemistry
  • Neuroscience
  • Clinical Diagnostics

Background:

  • Brain-derived neurotrophic factor (BDNF) is crucial for neuronal health.
  • Accurate measurement of BDNF in accessible tissues like blood is challenging.
  • Platelets are a potential source for BDNF quantification.

Purpose of the Study:

  • To develop and validate a sensitive method for measuring BDNF protein in human platelets.
  • To establish a reference concentration of BDNF in normal human platelets.
  • To assess the suitability of the method for clinical applications.

Main Methods:

  • A capture enzyme-linked immunosorbent assay (CELISA) was employed.
  • The assay was optimized for sensitivity and reproducibility.
  • BDNF concentration was measured in normal human blood platelets.

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Main Results:

  • The CELISA method provided accurate and reproducible measurements of platelet BDNF.
  • A mean concentration of 1.03 +/- 0.04 ng (SEM)/mg of platelet protein was observed.
  • The assay requires only 10 ml of blood.

Conclusions:

  • The developed CELISA is a reliable method for quantifying BDNF in human platelets.
  • This assay facilitates the investigation of BDNF levels in various clinical disorders.
  • The method's low blood requirement makes it clinically practical.