Related Experiment Video

Updated: Jun 29, 2026

Microvolume Protein Concentration Determination using the NanoDrop 2000c Spectrophotometer
10:29

Microvolume Protein Concentration Determination using the NanoDrop 2000c Spectrophotometer

Published on: November 5, 2009

Amperometric method for quantitative determination of proteins

E M Skobets', A P Megalins'ka

    Ukrains'Kyi Biokhimichnyi Zhurnal
    |November 1, 1977
    PubMed

    More Related Videos

    Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification09:04

    Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

    Published on: August 17, 2015

    Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method07:58

    Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method

    Published on: September 19, 2018

    Related Concept Videos

    Enzyme-Linked Immunosorbent Assay01:33

    Enzyme-Linked Immunosorbent Assay

    In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
    There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.
    Amperometry: Overview01:10

    Amperometry: Overview

    Amperometry is a technique commonly used to measure the concentration of specific analytes in a solution by monitoring the electric current generated during an electrochemical reaction. It involves applying a constant potential between a working electrode and a reference electrode to measure the resulting current, which is proportional to the concentration of the analyte. The Clark oxygen electrode operates based on this principle of amperometry. It consists of a cathode and an anode enclosed...
    Protein-Drug Binding: Determination Methods01:22

    Protein-Drug Binding: Determination Methods

    Determining protein-drug binding can be achieved through indirect and direct methods, each providing valuable insights into the interaction between proteins and drugs.
    Indirect methods involve isolating the bound drug from its free form in biological samples such as blood, serum, or plasma. These techniques aim to measure the percentage of drugs bound to proteins. Equilibrium dialysis is a commonly used method where the free drug concentration at equilibrium is measured by separating the bound...