Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Carboxypeptidases from different microbiolorganisms]

G S Piliavs'ka, O S Tsiperovich

    Ukrains'Kyi Biokhimichnyi Zhurnal
    |November 1, 1977
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [Effect of deuteration on some IR-spectral characteristics of gelatin].

    Ukrains'kyi biokhimichnyi zhurnal·1977
    Same author

    [Gelatin from bone collagen hydrolyzed with Streptomyces griseus protease and its properties].

    Ukrains'kyi biokhimichnyi zhurnal·1975
    Same author

    [Chymotrypsin-like proteinases in microbial proteolytic complexes].

    Ukrains'kyi biokhimichnyi zhurnal·1975
    Same journal

    Amperometric method for quantitative determination of proteins.

    Ukrains'kyi biokhimichnyi zhurnal·1977
    Same journal

    [On the potential protective properties of vitamin A in carcinogenesis].

    Ukrains'kyi biokhimichnyi zhurnal·1977
    Same journal

    [Stimulation of gluconeogenesis with 1,3-butanediol in the perfused rat liver].

    Ukrains'kyi biokhimichnyi zhurnal·1977
    Same journal

    [Isoenzymic spectra of lactate dehydrogenase and malate dehydrogenase in the loach unfertilized eggs and embryos].

    Ukrains'kyi biokhimichnyi zhurnal·1977
    Same journal

    [Intake dynamics of GABA and its pantoate derivative in mice brain areas].

    Ukrains'kyi biokhimichnyi zhurnal·1977
    Same journal

    [Peculiarities of the action of protein positively reacting in the sedimentation test for cancer on the activity of glycolytic enzymes].

    Ukrains'kyi biokhimichnyi zhurnal·1977
    See all related articles

    This study investigated alkali and acid carboxypeptidases in various microorganisms. Fungi primarily produce acid carboxypeptidases, while actinomycetes yield alkali carboxypeptidases, with Streptomyces griseus showing high activity.

    Area of Science:

    • Biochemistry
    • Microbiology
    • Enzymology

    Background:

    • Carboxypeptidases are crucial enzymes involved in protein degradation.
    • Understanding the distribution and characteristics of alkali and acid carboxypeptidases across different microbial groups is essential for biotechnological applications.

    Purpose of the Study:

    • To detect and characterize alkali and acid carboxypeptidases in a diverse range of microbial sources.
    • To compare the distribution patterns and production levels of these enzymes in fungi, yeast, actinomycetes, bacteria, and algae.

    Main Methods:

    • Enzymic preparations from 39 microbial sources were analyzed for the presence of alkali and acid carboxypeptidases.
    • Comparative analysis of enzyme distribution, activity, and stability was performed.

    Related Experiment Videos

    Main Results:

    • Significant variations in carboxypeptidase distribution were observed across microbial taxa.
    • Fungi predominantly synthesize acid carboxypeptidases (e.g., Aspergillus oryzae, Aspergillus flavus), while actinomycetes primarily produce alkali carboxypeptidases (e.g., Streptomyces griseus).
    • Streptomyces griseus exhibited exceptionally high specific activities for its carboxypeptidase preparations, surpassing previously studied enzymes, and demonstrated relative stability during purification.

    Conclusions:

    • Microbial source significantly influences the type and abundance of carboxypeptidases produced.
    • Actinomycetes, particularly Streptomyces griseus, represent a promising source for highly active and stable alkali carboxypeptidases.
    • The distinct enzymatic profiles of fungi and actinomycetes highlight their specialized roles in proteolytic processes.