Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

CD4+ T-cell testing practices as implications for training

E Rosner1, V Willis, R Lambert

  • 1Centers for Disease Control and Prevention, Public Health Practice Program Office, Division of Laboratory Systems, Chamblee, Georgia 30341, USA.

Cytometry
|August 15, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Serum adiponectin levels in patients with diffuse idiopathic skeletal hyperostosis (DISH).

Clinical rheumatology·2018
Same author

A model for the efficacy of combined inhibitors.

Journal of applied microbiology·2003
Same author

B-cell lymphoma developing in the donor 9 years after donor-origin acute myeloid leukemia post bone marrow transplantation.

Bone marrow transplantation·2003
Same author

Magnification and chromoscopy with the acetic acid test.

Endoscopy·2003
Same author

Treatment of esophagogastric tumors.

Endoscopy·2003
Same author

Food and carcinogenesis.

IARC scientific publications·2002
Same journal

Measurement of the distribution of red blood cell deformability using an automated rheoscope.

Cytometry·2002
Same journal

Flow cytometric-based isolation of nucleated erythroid cells during maturation: an approach to cell surface antigen studies.

Cytometry·2002
Same journal

Flow cytometric immunophenotyping test for staging/monitoring neuroblastoma patients.

Cytometry·2002
Same journal

Determination of bronchoalveolar lavage leukocyte populations by flow cytometry in patients investigated for respiratory disease.

Cytometry·2002
Same journal

Multicenter clinical experience with flow cytometric method for fetomaternal hemorrhage detection.

Cytometry·2002
Same journal

Separation of live cells in different phases of the cell cycle for gene expression analysis.

Cytometry·2002
See all related articles

Clinical laboratories performing CD4+ T-cell counts (CD4) generally follow recommended practices. However, some variations in quality control, quality assurance, and safety indicate potential training needs for laboratory personnel using flow cytometry.

Area of Science:

  • Clinical laboratory science
  • Immunology
  • Medical diagnostics

Background:

  • Emerging diseases and testing methods necessitate continuous skill updates for clinical laboratory personnel.
  • Complex techniques like flow cytometry demand significant education and practical experience for proficiency.
  • Assessing laboratory practices against expert guidelines identifies areas requiring targeted training.

Purpose of the Study:

  • To evaluate current laboratory practices for CD4+ T-cell counting.
  • To identify discrepancies between reported practices and established guidelines.
  • To establish a baseline for flow cytometry practices in 1993.

Main Methods:

  • A written survey was distributed to 206 laboratories performing CD4+ T-cell counts.

Related Experiment Videos

  • Participants selected their laboratory's practices from a list of alternatives for key testing steps.
  • Survey questions were informed by published guidelines, expert interviews, and focus groups.

    • Main Results:

    • A 73% response rate (247/337 laboratories) indicated general adherence to recommended practices.
    • Notable deviations were observed in quality control, quality assurance, and safety protocols.
    • The study provides a snapshot of flow cytometry practices in 1993.

    Conclusions:

    • Most laboratories adhere to recommended CD4+ T-cell testing procedures.
    • Specific areas, including quality control and safety, present opportunities for enhanced training.
    • The findings establish a benchmark for flow cytometry practices during the initial implementation phase of testing guidelines.