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Tandemly repeated 147 bp elements cause structural and functional variation in divergent MAL promoters of

P J Bell1, V J Higgins, I W Dawes

  • 1Burns Philp Research and Development, New South Wales, Australia.

Yeast (Chichester, England)
|September 25, 1997
PubMed

Insights

Novel repeats in yeast MAL promoters reduce maltose permease (MALT) expression more than maltase (MALS) expression. This finding suggests a regulatory mechanism to prevent potential toxicity from overexpressed permease genes.

Area of Science:

  • Molecular biology
  • Yeast genetics
  • Gene regulation

Background:

  • Yeast MAL regulon controls the metabolism of maltose.
  • Understanding gene regulation is crucial for metabolic engineering.

Purpose of the Study:

  • To investigate the function of novel tandem repeats found in yeast MAL promoters.
  • To determine the impact of these repeats on MALT and MALS gene expression.

Main Methods:

  • Isolation and characterization of four novel MAL promoters from Saccharomyces cerevisiae.
  • Deletion and insertion analyses of 147 bp repeats within MAL and CYC1 promoters.
  • Quantitative analysis of MALT and MALS gene expression levels.

Main Results:

  • Up to five tandem 147 bp repeats were identified in the studied MAL promoters.
  • These repeats significantly repressed MALT gene expression while only weakly affecting MALS expression.
  • The inhibitory effect was dependent on the repeat location between the Upstream Activating Sequence (UAS) and TATA box.

Conclusions:

  • The identified repeats function as negative regulatory elements in MAL promoters.
  • This repeat-mediated repression likely evolved to balance MALT and MALS expression, preventing MALT over-expression toxicity.
  • These findings provide insights into the complex regulatory networks governing yeast carbon source metabolism.

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