Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

DNA extraction from liquid blood using QIAamp

C A Scherczinger¹, M T Bourke, C Ladd

¹Connecticut State Police Forensic Science Laboratory, Meriden, USA.

Journal of Forensic Sciences

|September 26, 1997

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Fixation of distal radius fractures using AO titanium volar distal radius plate.

Hand surgery : an international journal devoted to hand and upper limb surgery and related research : journal of the Asia-Pacific Federation of Societies for Surgery of the Hand·2003

Same author

Screening for childhood obesity: international vs population-specific definitions. Which is more appropriate?

International journal of obesity and related metabolic disorders : journal of the International Association for the Study of Obesity·2003

Same author

Therapeutic effect of recombinant human erythropoietin on anaemia with erythropoietin deficiency in diabetic patients.

Diabetic medicine : a journal of the British Diabetic Association·2003

Same author

Communication skill of general practitioners: any room for improvement? How much can it be improved?

Medical education·2003

Same author

Relationship between changes in interferon-gamma production by peripheral blood T cells and changes in peak expiratory flow rate in patients with chronic stable asthma.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2003

Same author

Minimal model for genome evolution and growth.

Physical review letters·2003

Same journal

Correction to "The impact of institutional authority on forensic evidence evaluation by criminal justice professionals".

Journal of forensic sciences·2026

Same journal

Estimation of postmortem submersion interval based on microbial community composition in human remains recovered from aquatic environments.

Journal of forensic sciences·2026

Same journal

Prevalence of novel psychoactive substances in selected clinical urine specimens submitted for drug monitoring.

Journal of forensic sciences·2026

Same journal

GenoEye: A machine learning-based framework for the prediction of intermediate eye color phenotypes.

Journal of forensic sciences·2026

Same journal

Sharp force trauma analysis without animal bones: A proposal for sustainable and ethical bone proxies.

Journal of forensic sciences·2026

Same journal

Absolute dating of modern paper using <sup>14</sup>C bomb peak data of the paper fibers.

Journal of forensic sciences·2026

See all related articles

Forensic DNA databasing requires efficient DNA isolation. The QIAamp Blood Kit offers a cost-effective, high-yield method for purifying DNA from blood samples, suitable for PCR analysis.

Area of Science:

Forensic Science
Molecular Biology
Biotechnology

Background:

Increasing implementation of convicted felon DNA databases necessitates efficient DNA isolation methods.
Forensic laboratories require high-throughput, cost-effective DNA purification from liquid blood samples.

Purpose of the Study:

To evaluate the QIAGEN QIAamp Blood Kit/Tissue Kit for DNA isolation from blood samples.
To compare the QIAamp procedure with standard organic extraction methods for forensic DNA databasing.

Main Methods:

Utilized the QIAGEN QIAamp Blood Kit/Tissue Kit for DNA isolation.
Compared yield, quality, and suitability for enzymatic reactions against SDS-Proteinase K/organic extraction/microcon purification.

Main Results:

Related Experiment Videos

The QIAamp procedure yielded 2-4 times more DNA than the organic extraction method.
Purified DNA was of high molecular weight (20-30 kb), with minimal degradation.
DNA was suitable for direct use in PCR and RFLP analyses without further purification.

Conclusions:

The QIAamp DNA isolation procedure meets the requirements for high throughput, yield, quality, and speed for forensic DNA databasing.
This method is a cost-effective and efficient solution for preparing samples for DNA databases.