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Spectrophotometric determination of the positional specificity of nonspecific and 1,3-specific lipases
R N Farias1, M Torres, R Canela
1Departamento de Bioquímica de la Nutrición, Instituto Superior de Investigaciones Biológicas (CONICET-UNT), San Miguel de Tucumán, Argentina. farias@insibio.unt.edu.ar
Analytical Biochemistry
|November 5, 1997
Abstract:
Using commercially available thiosubstrates, such as 2,3-dimercapto-1-propanol tributyrate, the regio-specificities of 1,3-specific and nonspecific lipases was confirmed. The spectrophotometric test is a simple, rapid, and convenient alternative method to those previously reported for the characterization of the positional specificities of new lipases.