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Related Experiment Videos

Sphingosine-mediated electroporative DNA transfer through lipid bilayers

N I Hristova1, I Tsoneva, E Neumann

  • 1Institute of Biophysics, Bulg. Acad. Sci., Sofia, Bulgaria.

FEBS Letters
|November 5, 1997
PubMed
Summary

Cationic sphingosine enhances plasmid DNA (pDNA) adsorption and electroporation in lipid bilayers. This facilitates pDNA electrotransport through the bilayer, improving gene delivery to cells and tissues.

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Area of Science:

  • Biophysics
  • Molecular Biology
  • Nanotechnology

Background:

  • Lipid bilayers are crucial for cell membrane function.
  • Electroporation is a method for delivering molecules into cells.
  • Sphingolipids play roles in membrane structure and function.

Purpose of the Study:

  • To investigate the effect of cationic sphingosine on plasmid DNA (pDNA) adsorption and electroporation in planar lipid bilayers.
  • To explore the mechanism of pDNA electrotransport through lipid bilayers.
  • To provide a basis for optimizing electroporative gene delivery.

Main Methods:

  • Formation of planar lipid bilayers using diphytanoyl lecithin and D-sphingosine.
  • Adsorption of pDNA to the lipid bilayers.
  • Electroporation experiments monitoring conductance changes.

Related Experiment Videos

  • Analysis of voltage-dependent channel-like events.

    • Main Results:

    • Cationic sphingosine significantly enhanced pDNA adsorption to lipid bilayers.
    • Electroporation facilitated pDNA transfer, creating channel-like events with conductance of 1-7 pS at Vm >= +30 mV.
    • Evidence suggests electrotransport of pDNA mediated by transient DNA-lipid complexes within elongated hydrophilic pores.

    Conclusions:

    • Cationic sphingosine acts as a facilitator for pDNA adsorption and electroporation.
    • The study demonstrates a mechanism for pDNA electrotransport across lipid bilayers.
    • Findings support the optimization of electroporative delivery of DNA and oligonucleotides into cells and tissues.