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Hemoglobin E-beta thalassemia in Uttar Pradesh
1Department of Genetics, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow.
Objective:
To evaluate the molecular make up of hemoglobin E-Beta thalassemia to facilitate diagnosis, genetic counseling and prenatal diagnosis in Uttar Pradesh.
Design:
DNA analysis.
Setting:
Referred hemolytic anemia cases to Genetics OPD of a tertiary care center.
Subjects:
21 families of HbE-thalassemia of which 19 were of UP origin.
Methods:
The patient and obligate carriers in their families were evaluated at hematological, biochemical and molecular level. A total of 62 cases were evaluated which included the index cases and their family members. Red blood cell indices, osmotic fragility, hemoglobin electrophoresis, quantitation of fetal hemoglobin, HbA2/E, serum iron and total iron binding capacity estimation were carried out in all the blood samples. DNA analysis was done for HbE and beta thalassemia mutations.
Results:
The commonest, IVSI-5 (G-->C) mutation (57%) was found along with HbE mutation. Only 23/26 cases belonged to the group of common beta-thal mutations as described in literature.
Conclusion:
Establishment of antenatal diagnostic services is necessary in those parts of India where both these mutations are commonly seen.