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A conjugative linear plasmid in Streptomyces laurentii ATCC31255
C Kinoshita-Iramina1, M Kitahara, K Doi
1Microbial Genetics Division, Institute of Genetic Resources, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
Bioscience, Biotechnology, and Biochemistry
|October 27, 1997
Summary
Streptomyces laurentii
Area of Science:
- Microbiology
- Molecular Biology
- Genetics
Background:
- Streptomyces laurentii ATCC31255 harbors a linear DNA plasmid, pSLL.
- A cured strain exhibited reduced sporulation and antibiotic production, linked to a circular plasmid, pSLS.
- Plasmid pSLL is essential for normal host cell function.
Purpose of the Study:
- To investigate the role of plasmid pSLL in Streptomyces laurentii.
- To determine the interaction between plasmids pSLL and pSLS.
- To understand the impact of pSLL on host cell physiology.
Main Methods:
- Conjugation of pSLL into a pSLL-cured strain.
- Phenotypic analysis of spore formation and thiostrepton production.
- Comparison of wild-type and plasmid-modified strains.
Main Results:
- Plasmid pSLL was successfully reintroduced into the cured strain via conjugation.
- Reintroduction of pSLL restored spore formation and thiostrepton productivity.
- Plasmid pSLL mitigated the negative effects of pSLS on the host.
Conclusions:
- Plasmid pSLL plays a crucial role in Streptomyces laurentii.
- pSLL suppresses detrimental effects caused by the circular plasmid pSLS.
- pSLL is vital for maintaining host cell viability and productivity.