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Updated: Aug 19, 2026

Fluorescence in situ hybridization (FISH) Protocol in Human Sperm
Published on: September 1, 2009
Pregnancy after intracytoplasmic sperm injection with sperm from a man with a 47,XXY Klinefelter's karyotype
B Hinney1, M Guttenbach, M Schmid
1University of Goettingen, Germany. BHINNEY@MED.UNI-GOETTINGEN.DE
Objective:
To report the initiation of a pregnancy that was achieved by intracytoplasmic sperm injection (ICSI) with sperm from a patient with Klinefelter's syndrome.
Design:
Case report.
Setting:
University women's hospital IVF center.
Patient(S):
A couple with primary infertility and nonmosaic 47,XXY karyotype of the male partner.
Intervention(S):
Intracytoplasmic sperm injection after ovarian stimulation and transvaginal ultrasound-guided oocyte pick-up with sperm from a hypergonadotropic man with a nonmosaic 47,XXY karyotype.
Main Outcome Measure(S):
Clinical pregnancy.
Result(S):
Despite a 47,XXY karyotype in all 50 analyzed lymphocyte metaphases, the sperm of the patient led to a clinical pregnancy with the first attempt of ICSI and intrauterine transfer of three embryos. The pregnancy stopped developing in the ninth week. Cytogenetic investigation of the abortion material revealed a numerical normal 46,XXY karyotype.
Conclusion(S):
Sperm from a patient with hypergonadotropic nonmosaic Klinefelter's syndrome, when used for ICSI, can lead to a pregnancy.
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