Related Experiment Videos

Transfer of small YACs to E. coli as large circular plasmids

E Frengen¹, C Wu, P J de Jong

¹Biotechnology Centre of Oslo, University of Oslo, Norway. efrengen@biotek.uio.no

Genetic Analysis : Biomolecular Engineering

|July 1, 1997

Abstract:

We have designed a YAC circularization vector, pCIRC3, allowing enrichment of the YAC DNA by exonuclease digestion of the linear yeast chromosomes. Due to the presence of P1 replicon sequences in this vector, the circular YACs would replicate as PACs in Escherischia coli.

Related Concept Videos

Articles linked to this work by shared authors, journal, and citation graph.

[Quantitative characteristics of rare and endangered Castanopsis kawakamii population].

Ying yong sheng tai xue bao = The journal of applied ecology·2002

[Neural network based on modified simplex method and its application in studying forest self-thinning].

Ying yong sheng tai xue bao = The journal of applied ecology·2002

Histone tails modulate nucleosome mobility and regulate ATP-dependent nucleosome sliding by NURF.

Proceedings of the National Academy of Sciences of the United States of America·2001

[The intracellular signaling pathway initiated by ginsenosides in hematopoietic cells].

Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi·2001

[An epidemiological study on the factors influencing length of hospitalization for inpatients of burns].

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2001

Sublethal cerebral ischemia inhibits caspase-3 activation induced by subsequent prolonged ischemia in the C57Black/Crj6 strain mouse.

Neuroscience letters·2001

See all related articles