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Identification and cloning of differentially expressed genes
1Department of Pediatrics, University of Iowa, Iowa City 52245, USA. msoares@blue.weeg.uiowa.edu
Current Opinion in Biotechnology
|November 14, 1997
Summary
New gene expression profiling methods leverage comprehensive human and mouse gene resources. Hybridization techniques using cDNA microarrays offer powerful, high-throughput gene expression analysis.
Area of Science:
- Genomics
- Molecular Biology
- Bioinformatics
Background:
- Current methods for identifying differentially expressed genes are limited.
- The availability of near-complete sets of cDNA clones and sequences for human and mouse genes is increasing.
Purpose of the Study:
- To highlight the advantages of new gene expression profiling strategies.
- To emphasize the importance of completing gene identification efforts.
Main Methods:
- Utilizing complex complementary DNA (cDNA) or ribonucleic acid (RNA) probes.
- Employing cDNA microarrays on glass slides or chips for hybridization.
Main Results:
- Hybridization-based strategies are becoming increasingly advantageous for gene expression surveys.
- The effectiveness of these methods relies on the availability of comprehensive gene resources.
Conclusions:
- Future gene expression analysis will benefit from advanced microarray technologies.
- Continued efforts to identify all human and mouse genes are crucial for advancing high-throughput gene expression studies.