Related Experiment Videos
Crystallization and characterization of bovine liver glutamate dehydrogenase
P E Peterson1, J Pierce, T J Smith
1Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA.
Journal of Structural Biology
|November 14, 1997
Abstract:
Bovine liver glutamate dehydrogenase has been crystallized as an abortive complex with glutamic acid, NADH, and an inhibitor, GTP. Crystals of this complex were grown using the sitting drop vapor diffusion method with PEG 8000 as the precipitant and diffract to better than 2.5 A resolution. The crystals belong to the space group P2(1) with an entire enzyme hexamer in the crystallographic asymmetric unit. Self-rotation and self-Patterson functions clearly define the orientation and position of this hexameric enzyme.