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Related Experiment Videos

The Ca2+-sensing receptor: a target for polyamines

S J Quinn1, C P Ye, R Diaz

  • 1Department of Medicine, Brigham and Women's Hospital, Boston, Massachusetts 02115, USA.

The American Journal of Physiology
|November 14, 1997
PubMed
Summary

Polyamines like spermine act as agonists for the calcium-sensing receptor (CaR), influencing calcium levels and hormone secretion. This suggests the CaR in tissues like the brain may target polyamine actions.

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Pharmacology

Background:

  • The calcium-sensing receptor (CaR) is activated by extracellular calcium (Ca(o)) and found in various tissues, including the brain and intestine.
  • Polyamines are endogenous polyvalent cations with potential signaling roles.

Purpose of the Study:

  • To investigate polyamines as agonists for the CaR.
  • To explore the effects of polyamines on CaR-expressing cells and parathyroid hormone secretion.

Main Methods:

  • Transfection of human embryonic kidney (HEK-293) cells with the human CaR.
  • Measurement of intracellular calcium (Ca(i)) transients, inositol phosphate production, and cation channel activity.
  • Assessment of parathyroid hormone secretion from bovine parathyroid cells.

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Main Results:

  • Spermine stimulated Ca(i) transients in CaR-transfected HEK cells with varying EC50 values.
  • The potency order was spermine > spermidine >> putrescine.
  • Extracellular calcium modulated spermine's EC50, and spermine increased CaR sensitivity to Ca(o).
  • Spermine inhibited parathyroid hormone secretion, mimicking high Ca(o) effects.

Conclusions:

  • Polyamines, particularly spermine, function as effective agonists for the CaR.
  • The brain and other tissues may utilize the CaR to mediate the effects of endogenous polyamines.