Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The Ca2+-sensing receptor: a target for polyamines

S J Quinn1, C P Ye, R Diaz

  • 1Department of Medicine, Brigham and Women's Hospital, Boston, Massachusetts 02115, USA.

The American Journal of Physiology
|November 14, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Differential genioglossus muscle responses to changes in breathing route in awake people with and without obstructive sleep apnea.

Journal of applied physiology (Bethesda, Md. : 1985)·2026
Same author

Effect of Water Flow on Bond Strength of Zirconia to Tooth Structure.

Operative dentistry·2025
Same author

The influence of thermal and hypoxia induced habitat compression on walleye (Sander vitreus) movements in a temperate lake.

Movement ecology·2025
Same author

Pressure and cold pain threshold reference values in a pain-free older adult population.

British journal of pain·2024
Same author

The Effectiveness of Preventative Interventions to Reduce Mental Health Problems in at-risk Children and Young People: A Systematic Review of Reviews.

Journal of prevention (2022)·2024
Same author

Practitioner perspectives on the nature, causes and the impact of poor mental health and emotional wellbeing on children and young people in contact with children's social care: A qualitative study.

Child abuse & neglect·2024
Same journal

Blood coagulation in fish.

The American journal of physiology·2011
Same journal

Renal tubular reabsorption, metabolic utilization and isomeric fractionation of lactic acid in the dog.

The American journal of physiology·2010
Same journal

The inactivation of placental toxin by human serum.

The American journal of physiology·2010
Same journal

Adrenal function following ovariectomy in the rat.

The American journal of physiology·2010
Same journal

Capillary permeability; perfusion of frog and guinea pig hind limbs.

The American journal of physiology·2010
Same journal

Evaluation of protective measures against sunburn.

The American journal of physiology·2010
See all related articles

Polyamines like spermine act as agonists for the calcium-sensing receptor (CaR), influencing calcium levels and hormone secretion. This suggests the CaR in tissues like the brain may target polyamine actions.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Pharmacology

Background:

  • The calcium-sensing receptor (CaR) is activated by extracellular calcium (Ca(o)) and found in various tissues, including the brain and intestine.
  • Polyamines are endogenous polyvalent cations with potential signaling roles.

Purpose of the Study:

  • To investigate polyamines as agonists for the CaR.
  • To explore the effects of polyamines on CaR-expressing cells and parathyroid hormone secretion.

Main Methods:

  • Transfection of human embryonic kidney (HEK-293) cells with the human CaR.
  • Measurement of intracellular calcium (Ca(i)) transients, inositol phosphate production, and cation channel activity.
  • Assessment of parathyroid hormone secretion from bovine parathyroid cells.

Related Experiment Videos

Main Results:

  • Spermine stimulated Ca(i) transients in CaR-transfected HEK cells with varying EC50 values.
  • The potency order was spermine > spermidine >> putrescine.
  • Extracellular calcium modulated spermine's EC50, and spermine increased CaR sensitivity to Ca(o).
  • Spermine inhibited parathyroid hormone secretion, mimicking high Ca(o) effects.

Conclusions:

  • Polyamines, particularly spermine, function as effective agonists for the CaR.
  • The brain and other tissues may utilize the CaR to mediate the effects of endogenous polyamines.