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Evaluation of different lymphoid tissue sources for the construction of human immunoglobulin gene libraries
Y L Yip1, N J Hawkins, M A Clark
1Department of Biotechnology, University of NSW, Sydney, Australia.
Background:
Phage display technology allows the isolation of novel human monoclonal antibodies. The technology relies on the construction of a recombinant antibody library and its display on phage particles. The quality of an antibody library is affected by several factors including the size, diversity and source of immunoglobulin genes.
Objective:
The aim of the project was to determine the best tissue source for the construction of antibody libraries.
Study Design:
Three tissue sources were used in this study: peripheral blood mononuclear cells from a healthy donor, Epstein-Barr virus (EBV) transformed peripheral blood mononuclear cells and lymph node tissue from individuals with breast cancer. The quality of each tissue source was assessed using two criteria: (1) the number of mature and activated B cells in each source; (2) the amount of immunoglobulin heavy and light chain genes amplifiable by polymerase chain reaction (PCR).
Results:
EBV-transformed peripheral blood mononuclear cells and lymph node tissue were shown to contain more B cells than peripheral blood mononuclear cells. A relatively larger amount of immunoglobulin gene products could be amplified from EBV-transformed peripheral blood mononuclear cells and the lymph node. However, immunoglobulin containing gamma 1 chains could not be amplified from EBV-transformed mononuclear cells, and the resultant pattern of gene amplification suggests a possible selection bias.
Conclusion:
This study indicates that among the three tissue sources examined, lymph node tissue is the most suitable source for the construction of antibody libraries.