Related Experiment Video
Updated: Aug 8, 2026
Colon Ascendens Stent Peritonitis (CASP) - a Standardized Model for Polymicrobial Abdominal Sepsis
Published on: December 19, 2010
Lipopolysaccharide binding protein: a marker for intraperitoneal bacterial infection in patients with CAPD
K Schäfer1, R R Schumann, S Stöteknuel
1University Hospital Freiburg, Department of Nephrology, Germany.
Abstract:
During systemic infection, the serum lipopolysaccharide binding protein (LBP) binds to the lipid A component of bacterial endotoxins and facilitates its delivery to the CD 14 receptor on the cell surface of macrophages, where proinflammatory cytokines are released. There is no knowledge to date whether LBP is also present in the effluent of patients with continuous ambulatory peritoneal dialysis (CAPD)-associated peritonitis. We investigated the dialysis effluent of 37 patients with CAPD peritonitis for immunoreactive LBP, tumor necrosis factor (TNF)-alpha, and interleukin (IL)-1 beta and compared the findings with the cytokine levels in 20 noninfected CAPD patients. The mean +/- SEM concentrations of LBP, TNF-alpha, and IL-1 beta were significantly higher in the effluent of patients with peritonitis than in noninfected CAPD effluent. In comparison to controls (0.23 +/- 0.05 microgram/mL), LBP was 0.68 +/- 0.13 microgram/mL in the effluent of patients with CAPD-associated infectious peritonitis. For TNF-alpha, levels were 0.50 +/- 0.25 pg/mL in the control effluent versus 124.7 +/- 46.6 pg/mL in the effluent of peritonitis patients. For IL-1 beta the levels were 0.24 +/- 0.14 pg/mL in the control effluent and 71.23 +/- 17.53 pg/mL in the peritonitis patients. Our findings demonstrate that LBP is significantly elevated in the effluent of CAPD patients during an episode of CAPD-associated peritonitis and might be used as a marker of intraperitoneal bacterial infection.
Insights
Lipopolysaccharide binding protein (LBP) is elevated in the dialysis effluent of continuous ambulatory peritoneal dialysis (CAPD) patients experiencing peritonitis. This finding suggests LBP may serve as a valuable marker for detecting intraperitoneal bacterial infections in CAPD patients.
Area of Science:
- Nephrology
- Immunology
- Infectious Diseases
Background:
- Systemic infections involve lipopolysaccharide binding protein (LBP) delivering bacterial endotoxins to macrophages, triggering cytokine release.
- The presence and role of LBP in the dialysis effluent of continuous ambulatory peritoneal dialysis (CAPD)-associated peritonitis are unknown.
Purpose of the Study:
- To investigate the levels of LBP, tumor necrosis factor (TNF)-alpha, and interleukin (IL)-1 beta in the dialysis effluent of CAPD patients with peritonitis.
- To determine if LBP can serve as a marker for intraperitoneal bacterial infection in CAPD patients.
Main Methods:
- Analysis of dialysis effluent from 37 CAPD patients with peritonitis and 20 noninfected CAPD patients.
- Measurement of immunoreactive LBP, TNF-alpha, and IL-1 beta concentrations using quantitative methods.
Main Results:
- Significantly higher concentrations of LBP, TNF-alpha, and IL-1 beta were observed in the effluent of patients with peritonitis compared to noninfected controls.
- LBP levels were 0.68 +/- 0.13 microgram/mL in peritonitis patients versus 0.23 +/- 0.05 microgram/mL in controls.
- TNF-alpha levels were 124.7 +/- 46.6 pg/mL in peritonitis patients versus 0.50 +/- 0.25 pg/mL in controls.
- IL-1 beta levels were 71.23 +/- 17.53 pg/mL in peritonitis patients versus 0.24 +/- 0.14 pg/mL in controls.
Conclusions:
- LBP is significantly elevated in the dialysis effluent during CAPD-associated peritonitis.
- Elevated LBP in CAPD effluent may indicate an intraperitoneal bacterial infection.
- LBP shows potential as a diagnostic marker for CAPD-associated peritonitis.
