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The N-acetylgalactosamine and lactosamine specific lectin from Iris hybrida leaves
M Ferens-Sieczkowska1, M Orczyk-Pawiłowicz, B Morawiecka
1Institute of Biochemistry and Molecular Biology, University of Wrocław, Poland.
Acta Biochimica Polonica
|January 1, 1997
Abstract:
The lectin isolated from the leaves of Iris hybrida binds specifically N-acetyl-galactosamine and lactose. Its molecule consists of two identical subunits bound by disulfide bonds. The lectin is a glycoprotein containing about 12% of sugars. It binds asialoglycoproteins containing complex type sugar chains. The binding is reduced by half at the concentration of 0.15 to 0.40 mM of the galactose containing disaccharides irrespectively to a type of galactose isomer. This indicates rather broad specificity of I. hybrida leaf lectin.