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Related Experiment Videos

Variability of the NS(S) protein among Rift Valley fever virus isolates

A A Sall1, P M de A Zanotto, H G Zeller

  • 1Institut Pasteur, Dakar, Senegal.

The Journal of General Virology
|November 21, 1997
PubMed
Summary

Rift Valley fever virus (RVF) strains from Africa show genetic diversity. Phylogenetic analysis revealed two distinct lineages, Egyptian and sub-Saharan, based on the NS(S) protein gene.

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Area of Science:

  • Virology
  • Molecular Biology
  • Genetics

Background:

  • Rift Valley fever (RVF) virus poses a significant threat to public health and livestock.
  • Understanding RVF virus genetic diversity is crucial for effective disease control and surveillance.

Purpose of the Study:

  • To analyze the genetic variability of RVF virus strains using the NS(S) protein coding region.
  • To establish the phylogenetic relationships among diverse RVF virus isolates from Africa.

Main Methods:

  • RT-PCR amplification and sequencing of the NS(S) protein coding region from 18 RVF virus strains.
  • Phylogenetic analysis of nucleotide and amino acid sequences.

Main Results:

  • Sequence divergence ranged from 0 to 9.6% at the nucleotide level and 0 to 9.5% at the amino acid level.

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  • Phylogenetic analysis identified two major lineages: Egyptian and sub-Saharan.
  • Conserved cysteine residues and potential phosphorylation sites were identified in the NS(S) protein.

    • Conclusions:

    • The NS(S) gene provides valuable insights into RVF virus evolution and strain relatedness.
    • The identified lineages suggest distinct evolutionary paths for RVF virus in different African regions.
    • Further research is needed to elucidate the function of the NS(S) protein.