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Related Experiment Videos

Evidence for protein dolichylation

M Hjertman1, J Wejde, A Dricu

  • 1Department of Tumor Pathology, Karolinska Institutet, Stockholm, Sweden.

FEBS Letters
|December 31, 1997
PubMed
Summary
This summary is machine-generated.

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Researchers found evidence of proteins covalently modified with dolichol (dol) in human colon carcinoma cells. This discovery sheds light on novel post-translational modifications and protein structures.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Background:

  • Dolichol (dol) is a lipid essential for N-glycosylation.
  • The precise mechanisms of dol incorporation into cellular proteins remain incompletely understood.

Purpose of the Study:

  • To investigate the covalent attachment of dolichol to cellular proteins in human colon carcinoma cells.
  • To characterize the nature and properties of dolichol-modified proteins.

Main Methods:

  • Human colon carcinoma cells were labeled with [3H]dolichol.
  • Proteins were delipidated and subjected to proteolytic digestion.
  • High-performance liquid chromatography (HPLC) and mass spectrometry were used for purification and analysis.

Main Results:

Related Experiment Videos

  • Dolichol was found to be bound to cellular proteins of various molecular weights (5, 10, 27, 75, and >140 kDa).
  • Proteolytic products revealed a hydrophobic peak containing both dolichol and cysteine, distinct from GG-cys.
  • Mass spectrometry confirmed the presence of dolichol-like lipids released from proteins.

Conclusions:

  • The study provides strong evidence for the existence of proteins covalently modified with dolichol.
  • This suggests a novel post-translational modification pathway involving dolichol-protein conjugation.