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Characteristics of two major lectins from mungbean (Vigna radiata) seeds
K N Suseelan1, C R Bhatia, R Mitra
1Nuclear Agriculture Division, Bhabha Atomic Research Centre, Mumbai, India.
Two major lectins, MBL-I and MBL-II, were purified from Vigna radiata L. seeds using ion-exchange and gel filtration chromatography techniques. MBL-I was found to be a tetramer with native M.W. of 132 kDa and subunit M.W. of 33 kDa having alpha-galactosidase activity. MBL-II consisted of two monomeric lectins with M.W. of 94 kDa and 89 kDa which were associated mainly with beta-galactosidase activity. Both MBL-I and MBL-II are D-galactose-specific lectins.
Two major lectins, MBL-I and MBL-II, were purified from Vigna radiata L. seeds using ion-exchange and gel filtration chromatography techniques. MBL-I was found to be a tetramer with native M.W. of 132 kDa and subunit M.W. of 33 kDa having alpha-galactosidase activity. MBL-II consisted of two monomeric lectins with M.W. of 94 kDa and 89 kDa which were associated mainly with beta-galactosidase activity. Both MBL-I and MBL-II are D-galactose-specific lectins.