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A new improved way of making double-barrelled ion-selective micro-electrodes
S O Semb1, B Amundsen, O M Sejersted
1Institute for Experimental Medical Research, University of Oslo, Ullevål Hospital, Norway.
Acta Physiologica Scandinavica
|October 6, 1997
Abstract:
The methods published for fabrication of double-barrelled ion-selective micro-electrodes are often difficult to follow and give inconsistent results. We describe here a new improved way of making double-barrelled ion-selective micro-electrodes from theta-style glass capillaries that offers several refinements which improve reproducibility: (1) good control of silanization and (2) dry-bevelling to assure no inter-barrel silane contamination and to minimize the signal-to-noise ratio by increasing the tip diameter, without compromising the sharpness of the tip.