Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Extracellular-matrix gene expression during mouse submandibular gland development

S P Macauley1, R W Tarnuzzer, G S Schultz

  • 1Department of Oral Biology, University of Florida College of Medicine, Gainesville 32610, U.S.A.

Archives of Oral Biology
|June 1, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evaluation of the Effect of Source Geometry on the Output of Miniature X-ray Tube for Electronic Brachytherapy through Simulation.

Journal of biomedical physics & engineering·2018
Same author

Modulation of mouse sperm-egg interaction, early embryonic development and trophoblastic outgrowth by activated and unactivated macrophages.

Endocrine·2010
Same author

Twenty years of breast cancer in Iran: downstaging without a formal screening program.

Annals of oncology : official journal of the European Society for Medical Oncology·2010
Same author

Localization of P. gingivalis in preterm delivery placenta.

Journal of dental research·2009
Same author

Different tropism of adenoviruses and adeno-associated viruses to corneal cells: implications for corneal gene therapy.

Molecular vision·2008
Same author

Endometrial expression of epithelial neutrophil-activating peptide-78 during the menstrual cycle or in progestin-only contraceptive users with breakthrough bleeding and the influence of doxycycline therapy.

Human reproduction (Oxford, England)·2006
Same journal

Dental stem cell-derived extracellular vesicles for oral tissue regeneration: Mechanisms, engineering strategies, and clinical translation.

Archives of oral biology·2026
Same journal

Ferroptosis in the pathogenesis and therapeutic target of oral infectious diseases: A narrative review.

Archives of oral biology·2026
Same journal

Prognostic and diagnostic evaluation of HSPG2 gene expression in oral squamous cell carcinoma: A case-control study.

Archives of oral biology·2026
Same journal

Dental traits: From anthropological foundations to clinical implications.

Archives of oral biology·2026
Same journal

Surface properties of irradiated and non-irradiated dentin following pH cycling and fluoride toothpaste exposure: An in vitro study.

Archives of oral biology·2026
Same journal

CircSSRP1 regulates SMAD3 to affect proliferation and apoptosis of human embryonic palatal mesenchymal cells in nonsyndromic cleft lip with or without cleft palate by sponging miR-708-5p.

Archives of oral biology·2026
See all related articles

This study tracked extracellular matrix gene expression during mouse submandibular gland development. Key matrix molecules increased significantly by day 16, indicating their crucial role in salivary gland morphogenesis.

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Salivary gland development involves complex epithelial-mesenchymal interactions.
  • Extracellular matrix (ECM) molecules are critical for tissue morphogenesis and differentiation.

Purpose of the Study:

  • To quantify mRNA expression levels of specific ECM molecules during early mouse submandibular gland development.
  • To correlate ECM gene expression with key developmental stages.

Main Methods:

  • Quantitative competitive reverse transcription-polymerase chain reaction (RT-PCR) was used to measure mRNA expression.
  • Total RNA was isolated from fetal BALB/c mouse submandibular glands at different developmental time points.
  • A synthetic template (pMATRIX) facilitated precise quantification of gene expression.

Related Experiment Videos

Main Results:

  • Collagen alpha2(I), collagen alpha1(III), and lysyl oxidase mRNA levels showed an increasing trend by day 16 of fetal development.
  • Elastin mRNA was detectable only on day 16.
  • Fibronectin and laminin B2 mRNA were constitutively present, with peak abundance on day 16.

Conclusions:

  • The study successfully measured dynamic changes in ECM mRNA concentrations during salivary gland development.
  • Specific ECM components show differential expression patterns correlating with gland morphogenesis.
  • Quantitative RT-PCR with a synthetic template is a powerful tool for studying gene expression during development.