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Heme peroxidase activity measured in single mosquitoes identifies individuals expressing an elevated oxidase for
W G Brogdon1, J C McAllister, J Vulule
1Entomology Branch F-22, Centers for Disease Control and Prevention, Chamblee, GA 30341, USA.
Journal of the American Mosquito Control Association
|February 12, 1998
Abstract:
Optimum conditions are described for a simple, rapid, microplate-based assay that indirectly measures the differences in oxidase levels between individual susceptible, resistant, or induced mosquitoes. A small proportion (0.01-0.1) of a single mosquito is used, allowing multiple replicates of the oxidase assay. Cytochrome C is used as a positive control. The levels of oxidase found in sample populations of pyrethroid-susceptible, pyrethroid-resistant, and phenobarbital-induced Anopheles albimanus mosquitoes are characterized with the assay.