[The effect of immunoregulation of preparation of Mycobacterium vaccea by nitrogen oxide]
Related Experiment Videos
1National Institute for the Control of Pharmaceutical and Biological Products, Beijing.
Objective:
Preparation of M. vaccea on synthesis of nitrite [NO2-, an oxidative end product of nitric oxide (NO) synthesis] of peritoneal macrophages by different condition of animals.
Methods:
First, the BALB/c mice were immunized by preparation of M. vaccea and effect of synthesis of NO2- by murine peritoneal macrophage was detected. Second, guinea pigs intraperitonealy challenged with M. tuberculosis H37Rv were immunized by preparation of M. vaccea.
Results:
It was demonstrated that the effect of synthesis of NO2- of the murine peritoneal macrophage of the immunized mice was significantly higher than the unimmunized mice (P < 0.01) and synthesis of NO2- of peritoneal macrophage of challenged guinea pigs immunized with M. vaccea was significantly lower than that of challenged guinea pigs unimmunized with M. vaccea (P < 0.05).
Conclusion:
The preparation of M. vaccea can not only increase the effect of releasing of NO2- of the peritoneal macrophage, but also regulate the effect of immuno-reaction of the animals so as to reduce the damage of the tissue.